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To: Jennifer Carroll MacNeill, Minister for Health

Stop BOTOX Animal Testing

The Irish Anti-Vivisection Society calls on the Irish Government to enact legislation immediately to ban the LD50 Botulinum Toxin Potency Test for cosmetic procedures.

Why is this important?

Botox is the brand name of one of the products containing the nerve poison botulinum toxin. The toxin is used for medical and increasingly for cosmetic applications. A small injection causes facial wrinkles to disappear. But the price for the short term 'beauty' is paid by the suffering and death of thousands of mice.

Each batch is tested using the LD50 test before it reaches consumers. A sample is injected into the abdomen of mice. The mice suffer paralysis, impaired vision and respiratory distress. After three or four days of suffering, they die from suffocation.

The IAVS has supported the campaign 'Stop Botox animal testing' since 2009 to inform the public, to put pressure on the manufacturers still testing on animals as well as on authorities to speed up the validation and implementation of animal-free methods and to remove the LD50 mouse assay in the EU legislation.
Ireland

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Updates

2026-04-15 16:59:13 +0100

The Irish Anti-Vivisection Society and Sentient Rights Ireland will come together to mark World Day for Animals in Laboratories on Wednesday, April 22nd at 12:30pm in Dublin City Centre.

We encourage you to join us in commemorating this important day. If you’re unable to attend, you can still support the campaign by signing our petition and sharing this event with others.

https://fb.me/e/6ODK0iWvB

2026-01-15 15:00:47 +0000

IAVS statement on 2024 Statistics for Animal Experiments in Ireland
Thousands of mice continue to suffer severe poisoning to test cosmetic ‘botox’-type products
https://irishantivivisection.org/2025/12/24/2024-statistics-for-animal-experiments-in-ireland/

2024-12-22 19:15:31 +0000

IAVS statement on 2023 Statistics for Animal Experiments in Ireland 
Increase in deadly ‘Botox’ toxicity testing drives appalling 2023 rise in Irish animal experiments
https://irishantivivisection.org/2024/12/19/2023-statistics-for-animal-experiments-in-ireland/

2024-04-16 11:02:49 +0100

Please join us in commemorating World Day For Animals In Laboratories 2024.
If you can't make it, please share this event with friends and family.

https://fb.me/e/a9115jkqJ

Thank you for your support!

Date: Wednesday, 24th April 2024
Time: 13:00
Place: Opposite St Stephen's Green Shopping Centre, Dublin, D02 HX65, Ireland
(leaflets & posters will be provided)

2023-08-15 19:26:22 +0100

1,000 signatures reached

2023-08-15 17:07:57 +0100

500 signatures reached

2023-05-30 19:39:57 +0100

Irish campaigners in EU push to stop Botox tests:
https://irishantivivisection.org/2023/04/22/irish-campaigners-to-stop-botox-ld50-test/

2023-05-30 19:19:18 +0100

The ugly truth about Botox - Watch video here:
https://www.youtube.com/watch?v=doDnTpsx7Oc

2023-05-30 19:18:02 +0100

For more information on Botox testing in Ireland visit: https://irishantivivisection.org/project/botox/